Heterogeneity of HL-A Antigen Preparations Is Due to Variable Sialic Acid Content
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چکیده
منابع مشابه
Measurement of sialic acid content is insufficient to assess bioactivity of recombinant human erythropoietin.
Assessment of biological potency and its comparison with clinical effects are important in the quality control of therapeutic glycoproteins. Animal models are usually used for evaluating bioactivity of these compounds. However, alternative methods are required to simplify the bioassay and avoid ethical issues associated with animal studies. Negatively charged sialic acid residues are known to b...
متن کاملNatural immunity to Sindbis virus is influenced by host tissue sialic acid content.
Recent studies have shown that the sialic acid content of Sindbis virus influences both its ability to active the alternative pathway in vitro and its susceptibility to complement dependent clearance from the bloodstream in vivo. Other studies have shown that the sialic acid content of Sindbis virus is determined by the host in which it is propagated. Because individuals vary in their cell surf...
متن کاملSialic Acid Content of the Erythrocyte and of an Ascites
92.6 (19). Sialic acids have been found on the eryth rocyte membranes of a number of vertebrate species (5, 7, 192). Viral or bacterial neuramini dases, enzymes which cleave the a-ketosidic link age between a terminal sialic acid group and a sugar or sugar derivative (9), can liberate free sialic acid from such red cell membranes (5, 7). The surface of the mammalian erythrocyte is neg atively c...
متن کاملStructural decomposition and heterogeneity of commercial lipoteichoic Acid preparations.
Fractionation of commercial preparations of lipoteichoic acids (LTA) by hydrophobic interaction chromatography (HIC) and nuclear magnetic resonance spectroscopy revealed very inhomogeneous compositions and decomposition of the LTA structure: LTA content of the preparations averaged 61% for Streptococcus pyogenes, 16% for Bacillus subtilis, and 75% for Staphylococcus aureus. The decomposition wa...
متن کاملMeasurement of sialic acid content on recombinant membrane proteins
Background Membrane proteins such as cell adhesion molecules, receptors, transporters and ion channel proteins all have essential roles in cell-growth, migration, and flow of information, cell-cell and cell-protein communication. Membrane proteins are targets of biopharmaceutical companies because they have diverse effects on the progression of many diseases [1]. Ion channels are membrane prote...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1974
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.71.10.3998